Human ubiquitin C-terminal hydrolase L1elisa technical specification

**Human Ubiquitin C-Terminal Hydrolase L1 (UCH-L1) ELISA Kit – Technical Specification** **Purpose:** This ELISA kit is designed for the quantitative determination of Human Ubiquitin C-Terminal Hydrolase L1 (UCH-L1) in human serum, plasma, and other biological fluids. It provides a reliable and sensitive method to measure UCH-L1 levels, which can be useful in clinical research and diagnostic applications. **Experimental Principle:** The assay is based on the double-antibody sandwich ELISA technique. A microtiter plate is pre-coated with a specific monoclonal antibody against UCH-L1. After incubation with the sample, UCH-L1 present in the sample binds to the immobilized antibody. A horseradish peroxidase (HRP)-labeled secondary antibody is then added, forming a complex of antibody-antigen-enzyme-labeled antibody. Following a washing step, the substrate TMB is added, and the reaction is stopped with a stop solution. The color intensity, measured at 450 nm, correlates directly with the concentration of UCH-L1 in the sample. A standard curve is used to calculate the exact concentration of UCH-L1 in the tested samples. **Kit Components:** 1. 30× Wash Buffer – 20 ml × 1 bottle 2. Enzyme Standard – 6 ml × 1 bottle 3. Enzyme-Labeled Coating Plate – 12 wells × 8 strips 4. Sample Diluent – 6 ml × 1 bottle 5. Developer A – 6 ml × 1 bottle 6. Developer B – 6 ml × 1 bottle 7. Stop Solution – 6 ml × 1 bottle 8. Standard (80 pg/ml) – 0.5 ml × 1 bottle 9. Standard Diluent – 1.5 ml × 1 bottle 10. Instruction Manual – 1 copy 11. Plate Sealing Film – 2 sheets 12. Aluminum Bag – 1 piece **Sample Requirements:** Samples should be processed as soon as possible after collection. For optimal results, it is recommended to perform the test immediately after sample preparation. If immediate testing is not feasible, store the samples at -20°C. Avoid repeated freeze-thaw cycles. Note that samples containing sodium azide (NaN3) are not suitable for this assay, as NaN3 may inhibit the activity of horseradish peroxidase (HRP), leading to inaccurate results. This ELISA kit ensures high specificity and sensitivity, making it ideal for research and diagnostic purposes related to UCH-L1 detection. Proper handling and storage of both reagents and samples are essential for obtaining accurate and reproducible results.

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