Effect of ultrapure water on high performance liquid phase (HPLC) experiment
Abstract: With the development of the times and science and technology, high-precision liquid phase and other precision analytical instruments have been popularized in industries related to analysis and testing, and the requirements for experimental water quality are becoming higher and higher. Nowadays, high-performance liquid phase (HPLC) requires users to use ultrapure water to set a flat baseline without peaks to ensure the sensitivity of analysis and testing, but some HPLC users still use bottled pure water or distilled water for experiments due to funds or habits. The effect is often not ideal.
Bottled pure water and distilled water are just ordinary pure water instead of high-purity water. The water still contains a small amount of impurities such as ions and organic matter. The preparation of the liquid phase and eluent may not only foul expensive chromatographic columns, but also affect HPLC to set a flat baseline .
The purification process of bottled pure water usually includes adsorption, filtration, reverse osmosis, etc. The removal of particulate organic matter is better, but the removal of trace organic matter cannot meet the needs of high-sensitivity HPLC experiments, and because its packaging is usually Most of them are PVC bottles with high oxygen permeability and a certain amount of chemical dissolution, which contaminates the pure water in the bottle, especially after a period of storage, the water quality drops more, so it is shown on the HPLC chromatogram, although there is no specific absorption Peak, but the baseline has more severe unstable interference.
Some experts found that 254nm and 214nm had strong absorption peaks at 22-27 minutes when they were tested by HPLC on double-distilled water, which indicates that there is a strong hydrophobic organic pollution, the reason should be the azeotropic phenomenon in the distillation process This has led to incomplete removal of certain volatile organic compounds.
"Water Purifier" ultra-pure water system integrates a variety of precision purification processes such as water quality pretreatment, double-stage reverse osmosis, and ultra-purification post-treatment. The water quality index of the produced water is close to Milli-Q water, and the ultra-pure water is ready to use, not Pollution will be introduced due to storage, and the water quality is guaranteed. Compared with the use of bottled pure water or distilled water, it can meet the needs of users with high-precision instrument analysis.
(Remarks: The higher the purity of water, the more susceptible to external pollution caused by air and containers. Ultra-pure water should be used immediately. In this way, the influence of external interference can be excluded as much as possible in the analysis of high-precision instruments. So as to get satisfactory results.)
"Water Purifier" ultra-pure water system removal process introduction:
(1) The activated carbon filter element contained in the "Water Purifier" water quality pre-processor can absorb and filter 80% of organic impurities in tap water.
(2) The RO reverse osmosis membrane pore size is as small as 0.001 microns, and the removal rate of organic matter with a molecular weight greater than 300dalton is more than 99%. The specially designed double-stage reverse osmosis organic matter content in the "Water Purifier" ultra-pure water system can be as low as 10-20ppb To meet the source water quality requirements of imported ultra-pure water machines such as Milli-Q.
(3) The "Water Purifier" ultra-pure water system specially designed UV digestion device can generate 185nm UV light generated hydroxyl radicals in water, can quickly oxidize a small amount of residual organic matter in water to carbon dioxide, water and some organic intermediate products Break.
(4) "Water Purifier" terminal microfilter can filter particulate organic matter and resin debris.
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