Mouse α1 acid glycoprotein (α1-AGP) elisa kit instruction manual

**Mouse α1 Acid Glycoprotein (α1-AGP) ELISA Kit Instruction Manual** **Kit Specifications:** This Mouse α1-Acid Glycoprotein (α1-AGP) ELISA Kit is available in two configurations: 48-well and 96-well formats. The kit includes all necessary reagents for quantitative detection of α1-AGP in biological samples such as serum, plasma, or cell culture supernatants. **Standard Diluent:** 1.5 ml × 1 bottle **Enzyme Standard Reagent:** 3 ml × 1 bottle (for 48-well) / 6 ml × 1 bottle (for 96-well) **Important Note:** This reagent is intended for research use only. It is not suitable for diagnostic or clinical purposes. **Procedure for Standard Curve Construction:** 1. Plot the standard curve by using the concentration of the standard on the x-axis and the corresponding OD values on the y-axis. 2. Draw a standard curve on graph paper or using software. 3. Determine the sample concentration by matching the OD value of the sample to the standard curve. 4. Multiply the calculated concentration by the dilution factor to obtain the actual sample concentration. Alternatively, calculate the linear regression equation from the standard curve data (concentration vs. OD). Use this equation to calculate the sample concentration based on its OD value, then multiply by the dilution factor to determine the real concentration. **Kit Components:** - Sealing Film: 2 pieces (for 48-well) / 2 pieces (for 96-well) - Standard: 2700 ng/L, 0.5 ml × 1 bottle - Sample Diluent: 3 ml × 1 bottle (for 48-well) / 6 ml × 1 bottle (for 96-well) - Enzyme Standard: 1×48 / 1×96 - Developer A: 3 ml × 1 bottle (for 48-well) / 6 ml × 1 bottle (for 96-well) - Chromogen B Solution: 3 ml × 1 bottle (for 48-well) / 6 ml × 1 bottle (for 96-well) - Stop Solution: 3 ml × 1 bottle (for 48-well) / 6 ml × 1 bottle (for 96-well) - Concentrated Wash Solution: (20 ml × 20 times) × 1 bottle (for 48-well) / (20 ml × 30 times) × 1 bottle (for 96-well) **Storage Conditions:** All components should be stored at 2–8°C, except for the Chromogen B solution, which should be stored at -20°C. **Precautions:** - Ensure all reagents are brought to room temperature before use. - Avoid contamination during the assay process. - Follow the instructions carefully to ensure accurate results. - Do not mix reagents from different kits or batches. This ELISA kit provides a reliable and sensitive method for measuring α1-AGP levels in various biological samples. It is ideal for researchers conducting studies related to inflammation, stress response, and other physiological processes involving acute-phase proteins.

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