Mouse α1 acid glycoprotein (α1-AGP) elisa kit instruction manual

**Mouse α1 Acid Glycoprotein (α1-AGP) ELISA Kit Instruction Manual** **Kit Specification:** This Mouse α1 Acid Glycoprotein (α1-AGP) ELISA Kit is available in 48-well or 96-well configurations. It is designed for quantitative detection of α1-AGP in biological samples, such as serum, plasma, and cell culture supernatants. **Kit Components:** - **Standard Diluent:** 1.5 ml × 1 bottle - **Enzyme Standard Reagent:** 3 ml × 1 bottle (for 48-well) / 6 ml × 1 bottle (for 96-well) - **Sealing Film:** 2 pieces (for 48-well) / 2 pieces (for 96-well) - **Standard:** 0.5 ml × 1 bottle, concentration: 2700 ng/mL - **Sample Diluent:** 3 ml × 1 bottle (for 48-well) / 6 ml × 1 bottle (for 96-well) - **Developer A:** 3 ml × 1 bottle (for 48-well) / 6 ml × 1 bottle (for 96-well) - **Chromogen B Solution:** 3 ml × 1 bottle (for 48-well) / 6 ml × 1 bottle (for 96-well) - **Stop Solution:** 3 ml × 1 bottle (for 48-well) / 6 ml × 1 bottle (for 96-well) - **Concentrated Washing Solution:** 20 ml × 20 times (for 48-well) / 20 ml × 30 times (for 96-well) **Storage Conditions:** All reagents should be stored at 2–8°C, except for the Chromogen B solution, which should be stored at -20°C to maintain stability. **Usage Instructions:** This kit is intended for research use only. To determine the concentration of α1-AGP in your sample, follow these steps: 1. Prepare the standard curve by plotting the known concentrations of the α1-AGP standards on the x-axis and their corresponding OD values (measured at 450 nm) on the y-axis. 2. Draw a standard curve using graph paper or software. 3. Measure the OD value of your sample and locate it on the standard curve to find the corresponding concentration. 4. Multiply this value by the dilution factor if the sample was diluted before testing. 5. Alternatively, calculate the linear regression equation (y = mx + b) from the standard curve and plug in the OD value of the sample into the equation to determine the concentration. **Notes:** - Ensure all reagents are brought to room temperature before use. - Avoid contamination during the procedure. - Perform the assay in duplicate to ensure accuracy. - Follow the manufacturer’s instructions carefully for optimal results. **Important:** This product is not for diagnostic or therapeutic use. Always handle reagents with care and follow proper laboratory safety protocols.

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