Analysis of the cause of the ELISA kit standard overflow
The ELISA procedure is cumbersome and the problems that the operators make are different. In the afternoon, Mr. Zhao from Liaoning University of Traditional Chinese Medicine contacted Shanghai Hengyuan He Manager to ask: Why does the ELISA standard product have an “overflow†phenomenon? After seeing the problem, Manager He immediately transferred to the technical department, 5 minutes later. The technical department of Shanghai Hengyuan Company sent a phone call to Teacher Zhao to analyze the reasons for the overflow of the standard value of the ELISA kit:
1 Sample/standard is not diluted correctly.
2 Incubation time / temperature is incorrect.
3 Cover the enzyme-coated plate cover during incubation: Cover the enzyme-coated plate cover during incubation to prevent contamination during liquid evaporation or incubation. There is a sufficient number of enzyme-linked plate covers in each kit.
4 The detection wavelength is not correct: Make sure that the detection wavelength of the spectrophotometer is correct.
5 TMB substrate color development time is too long: please monitor the color development time. The color development time shown in the specification may vary slightly depending on the temperature and other detection conditions.
6 Samples are not suitable for this test: The applicable specimen range for each product is listed in the instruction manual. Specimens that are out of range may not be suitable for the kit or the test conditions need to be explored.
7 Sample/Standard Contamination: Contamination of samples or standards may result in “overflow†of test results.
8 Improper dilution of coupling reagent: Dilute the coupling reagent strictly according to the instructions. If the concentration is too high, it will cause incorrect dilution of OD “overflow†HRP coupling reagent.
9 The diluent used is incorrect: only dilute the sample and standard with the diluent in the appropriate kit.
After finding the cause of the problem, it is much easier to solve the problem. The same is true for all of you, you must be careful when testing, and strictly follow the requirements. Read the instructions of the ELISA kit carefully before the experiment. Shanghai Hengyuan wish you all a successful experiment!
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