Separate pure culture method with solid medium
A single microorganism grows on the surface or inside of a suitable solid medium and multiplies to a certain degree to form a subpopulation of child cells that is visible to the naked eye and has a certain morphological structure, called a colony. When many colonies on the surface of the solid medium are combined into one piece, it becomes a lawn. Different microorganisms grow on a specific medium to form colonies or mosses, which generally have stable characteristics, which can become an important basis for classification and identification of the microorganisms. Most bacteria, yeasts, as well as many fungi and single-cell algae can form isolated colonies on the solid medium, and it is easy to obtain pure culture using the appropriate plate separation method. The so-called plate refers to the abbreviation of culture plate. It refers to a plate containing molten solid medium poured into a sterile plate and cooled and solidified. This method involves separating and fixing individual microorganisms on or in the solid medium. A solid medium is a medium solidified with agar or other gel substances. Each isolated living microorganism grows and reproduces to form colonies, and the formed colonies are easy to transplant. The most commonly used solid medium for separation and culture is an agar solid medium plate. This technology established by Koch, which uses a plate to separate microorganisms for pure culture, is simple and easy to use. It has been the most commonly used method for the separation of various bacterial species for more than 100 years.
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