Human 8-hydroxydeoxyguanosine (8-OHdG) enzyme-linked immunoassay (ELISA)
Human 8-hydroxydeoxyguanosine (8-OHdG) enzyme-linked immunoassay (ELISA)
Kit instruction manual
This reagent is for research purpose only: this kit is used to determine the content of 8-hydroxydeoxyguanosine (8-OHdG) in human serum, plasma and related liquid samples.
Experimental principle:
This kit uses the double antibody sandwich method to determine the level of human 8-hydroxydeoxyguanosine (8-OHdG) in the specimen. Microporous plates were coated with purified human 8-hydroxydeoxyguanosine (8-OHdG) antibody to make solid-phase antibodies. 8-hydroxydeoxyguanosine (8-OHdG) was added to the microwells coated with mAb in turn HRP-labeled 8-hydroxydeoxyguanosine (8-OHdG) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with human 8-hydroxydeoxyguanosine (8-OHdG) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human 8-hydroxydeoxyguanosine (8-OHdG) in the sample was calculated by a standard curve.
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